3J0J

Fitted atomic models of Thermus thermophilus V-ATPase subunits into cryo-EM map

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	V-type ATP synthase alpha chain	polymer	578	63700.0	3	UniProt (Q56403) Pfam (PF02874) Pfam (PF16886) Pfam (PF00006) Pfam (PF22919)	Thermus thermophilus	V-ATPase subunit A
2	D, E, F (D, E, F)	V-type ATP synthase beta chain	polymer	478	53219.5	3	UniProt (Q56404) Pfam (PF02874) Pfam (PF00006) Pfam (PF22919)	Thermus thermophilus	V-ATPase subunit B
3	G (G)	V-type ATP synthase subunit D	polymer	223	24715.6	1	UniProt (O87880) Pfam (PF01813)	Thermus thermophilus	V-ATPase subunit D
4	H (H)	V-type ATP synthase subunit F	polymer	104	11294.9	1	UniProt (P74903) Pfam (PF01990)	Thermus thermophilus	V-ATPase subunit F
5	I, K (I, K)	V-type ATP synthase, subunit (VAPC-THERM)	polymer	104	11621.4	2	UniProt (Q5SIT5) Pfam (PF16999)	Thermus thermophilus
6	J, L (J, L)	V-type ATP synthase subunit E	polymer	188	20699.7	2	UniProt (P74901) Pfam (PF01991)	Thermus thermophilus	V-ATPase subunit E
7	M (M)	V-type ATP synthase subunit C	polymer	323	35968.6	1	UniProt (P74902) Pfam (PF01992)	Thermus thermophilus	V-ATPase subunit C
8	N, O (A, C)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	2	Chemie (ADP)

Sequence modifications

I, K: 18 - 120 (UniProt: Q5SIT5)

PDB	External Database	Details
Gly 17	-	expression tag

J, L: 1 - 188 (UniProt: P74901)

PDB	External Database	Details
Met 134	Leu 134	conflict
Met 171	Leu 171	conflict
Met 178	Leu 178	conflict

Contents of the asymmetric unit

Polymers	Number of chains	13
	Total formula weight	487379.6
Non-Polymers*	Number of molecules	2
	Total formula weight	854.4
All*	Total formula weight	488234.0

*Water molecules are not included.