3J0J
Fitted atomic models of Thermus thermophilus V-ATPase subunits into cryo-EM map
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK III |
| Method | Blot 20 seconds before plunging |
EM Data Collection
| Microscope | FEI TECNAI F20 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2 |
| Detector | GENERIC FILM |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 9.7 |
| Software | iMODFIT,UCSF Chimera,Build_Fspace,FREALIGN,Refine_Fspace |
| Symmetry | C1 |
