3IZ0

Human Ndc80 Bonsai Decorated Microtubule

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	alpha tubulin, Chain A from PDB 1JFF	polymer	451	50107.2	1	Pfam (PF00091) Pfam (PF03953) UniProt (by SIFTS) (P81947) In PDB	Bos taurus
2	B	beta tubulin, Chain B from PDB 1JFF	polymer	445	49907.8	1	Pfam (PF00091) Pfam (PF03953) UniProt (by SIFTS) (Q6B856) In PDB	Bos taurus
3	C, E	NDC80-SPC25 chimera protein, Chain B from PDB 2VE7 (Ndc80 bonsai)	polymer	315	36351.3	2	UniProt (Q05DQ6) UniProt (Q9HBM1) Pfam (PF03801) Pfam (PF18077) Pfam (PF08234) UniProt (by SIFTS) (O14777) In PDB	Homo sapiens (human)
4	D, F	NUF2-SPC24 chimera protein, Chain D from PDB 2VE7 (Ndc80 bonsai)	polymer	250	28993.4	2	UniProt (B1AQT4) UniProt (C9JGC4) Pfam (PF03800) Pfam (PF08286) UniProt (by SIFTS) (Q9BZD4) UniProt (by SIFTS) (Q8NBT2) In PDB	Homo sapiens (human)
5	A	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
6	A	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
7	A	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	1	Chemie (GTP)
8	B	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	1	Chemie (GDP)
9	B	TAXOL	non-polymer		853.9	1	Chemie (TA1)

Sequence modifications

C, E: 80 - 286 (UniProt: Q05DQ6)

PDB	External Database	Details
Met 79	-	expression tag

C, E: 287 - 393 (UniProt: Q9HBM1)

PDB	External Database	Details
Gln 393	Asn 224	conflict

D, F: 1 - 169 (UniProt: B1AQT4)

PDB	External Database	Details
Gly -4	-	expression tag
Pro -3	-	expression tag
Leu -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Gly 72	Glu 72	engineered mutation

D, F: 170 - 245 (UniProt: C9JGC4)

PDB	External Database	Details
Glu 200	Asp 152	conflict

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	230704.4
Non-Polymers*	Number of molecules	5
	Total formula weight	1910.0
All*	Total formula weight	232614.4

*Water molecules are not included.