3IZ0

Human Ndc80 Bonsai Decorated Microtubule

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	alpha tubulin, Chain A from PDB 1JFF	polymer	451	50107.2	1	Pfam (PF00091) Pfam (PF03953) UniProt (by SIFTS) (Q2HJ86)	Bos taurus
2	B (B)	beta tubulin, Chain B from PDB 1JFF	polymer	445	49907.8	1	Pfam (PF00091) Pfam (PF03953) UniProt (by SIFTS) (Q6B856)	Bos taurus
3	C, E (C, E)	NDC80-SPC25 chimera protein, Chain B from PDB 2VE7 (Ndc80 bonsai)	polymer	315	36351.3	2	UniProt (Q05DQ6) UniProt (Q9HBM1) Pfam (PF03801) Pfam (PF18077) Pfam (PF08234) UniProt (by SIFTS) (O14777)	Homo sapiens (human)
4	D, F (D, F)	NUF2-SPC24 chimera protein, Chain D from PDB 2VE7 (Ndc80 bonsai)	polymer	250	28993.4	2	UniProt (B1AQT4) UniProt (C9JGC4) Pfam (PF03800) Pfam (PF08286) UniProt (by SIFTS) (Q9BZD4) UniProt (by SIFTS) (Q8NBT2)	Homo sapiens (human)
5	G (A)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
6	H (A)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG) PubChem (888)
7	I (A)	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	1	Chemie (GTP) PubChem (177679032) PubChem (135400627) PubChem (135487750) PubChem (136088935) PubChem (136803486) PubChem (135398633) PubChem (135544356) PubChem (135547949) PubChem (135600366) PubChem (135749822) PubChem (136005188) PubChem (136073923) PubChem (136240914) PubChem (136664747) PubChem (136772743) PubChem (136772744) PubChem (136803487) PubChem (137286749) PubChem (164715984) PubChem (171699466)
8	J (B)	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	1	Chemie (GDP) PubChem (136073927) PubChem (135398619) PubChem (135402030) PubChem (135431654) PubChem (135566337) PubChem (135760556) PubChem (136026707) PubChem (136053418) PubChem (136663964) PubChem (136664748) PubChem (136759848) PubChem (136759849) PubChem (136759850) PubChem (136836636) PubChem (136847074) PubChem (172898537)
9	K (B)	TAXOL	non-polymer		853.9	1	Chemie (TA1) PubChem (16757828) PubChem (16760674) PubChem (21458255) PubChem (23307219) PubChem (23412627) PubChem (23636632) PubChem (24848897) PubChem (24867246) PubChem (16760177) PubChem (16742549) PubChem (21733096) PubChem (23307242) PubChem (23307253) PubChem (23307913) PubChem (24754091) PubChem (24207713) PubChem (24791027) PubChem (25200067) PubChem (42613129) PubChem (42620039) PubChem (44155032) PubChem (44276363) PubChem (44279927) PubChem (44335735) PubChem (44291178) PubChem (44328863) PubChem (44342632) PubChem (44356895) PubChem (44378736) PubChem (45489039) PubChem (46782671) PubChem (71209844) PubChem (53313247) PubChem (53298534) PubChem (54259297) PubChem (54259299) PubChem (57489669) PubChem (57999380) PubChem (58460861) PubChem (58777027) PubChem (58798493) PubChem (58596717) PubChem (59048611) PubChem (59042813) PubChem (58877402) PubChem (44323108) PubChem (44356961) PubChem (44403716) PubChem (60005248) PubChem (59873251) PubChem (59914794) PubChem (59919147) PubChem (60123436) PubChem (60011010) PubChem (60147315) PubChem (66795656) PubChem (14060298) PubChem (46781469) PubChem (176586897) PubChem (177678861) PubChem (36314) PubChem (60915) PubChem (177409) PubChem (184492) PubChem (70801836) PubChem (70842048) PubChem (70848451) PubChem (70856170) PubChem (441276) PubChem (73707502) PubChem (73759704) PubChem (89493063) PubChem (89847578) PubChem (90057663) PubChem (91122402) PubChem (91885464) PubChem (49868078) PubChem (5317123) PubChem (5321715) PubChem (101920282) PubChem (5352019) PubChem (121459571) PubChem (122361554) PubChem (6604028) PubChem (6604616) PubChem (6604814) PubChem (6713921) PubChem (6915727) PubChem (124747272) PubChem (124893098) PubChem (9875988) PubChem (54259298) PubChem (126968948) PubChem (129395998) PubChem (129627423) PubChem (10748131) PubChem (132517454) PubChem (11320430) PubChem (11354895) PubChem (134368714) PubChem (134972678) PubChem (137705323) PubChem (138106885) PubChem (140731501) PubChem (140512017) PubChem (146034004) PubChem (153289350) PubChem (154037993) PubChem (57908851) PubChem (57946364) PubChem (155982214) PubChem (159121804) PubChem (160053861) PubChem (159716711) PubChem (160171678) PubChem (160585530) PubChem (163077211) PubChem (163077212) PubChem (163077209) PubChem (163284947) PubChem (58803237) PubChem (168322423) PubChem (169917882) PubChem (171036874) PubChem (175758871) PubChem (59844545) PubChem (59904698) PubChem (60123443) PubChem (70943371) PubChem (71096818) PubChem (73759783) PubChem (101598423) PubChem (118528174) PubChem (4666) PubChem (124747271) PubChem (124747273) PubChem (124747274) PubChem (5460441) PubChem (126761000) PubChem (9854162) PubChem (9897470) PubChem (10033513) PubChem (10485732) PubChem (11506104) PubChem (11828953) PubChem (14060299) PubChem (14060304) PubChem (14060306) PubChem (14060309) PubChem (15983941) PubChem (133052515) PubChem (133640187) PubChem (134715514) PubChem (143890766) PubChem (152743329) PubChem (154828192) PubChem (144453087) PubChem (160585532) PubChem (163077210) PubChem (163077213) PubChem (163077214) PubChem (163077215) PubChem (165084161) PubChem (166711459) PubChem (167423928) PubChem (170014955) PubChem (170362150)

Sequence modifications

C, E: 80 - 286 (UniProt: Q05DQ6)

PDB	External Database	Details
Met 79	-	expression tag

C, E: 287 - 393 (UniProt: Q9HBM1)

PDB	External Database	Details
Gln 393	Asn 224	conflict

D, F: 1 - 169 (UniProt: B1AQT4)

PDB	External Database	Details
Gly -4	-	expression tag
Pro -3	-	expression tag
Leu -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Gly 72	Glu 72	engineered mutation

D, F: 170 - 245 (UniProt: C9JGC4)

PDB	External Database	Details
Glu 200	Asp 152	conflict

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	230704.4
Non-Polymers*	Number of molecules	5
	Total formula weight	1910.0
All*	Total formula weight	232614.4

*Water molecules are not included.