3IZ0

Human Ndc80 Bonsai Decorated Microtubule

EM specimen

Vitrification	Yes
Instrument	FEI VITROBOT MARK I
Method	2ul of 0.25 mg per ml MTs applied to grid for 1 minute 4ul of 0.7 mg per ml Ndc80 bonsai added, 1 minute manually blotted, then another 4ul of Ndc80 applied 1 minute 2ul removed with pipetter Blot for 2 seconds before plunging, 0mm offset

EM Data Collection

Microscope	FEI TECNAI F20
Electron Gun	FIELD EMISSION GUN
Accelerating Voltage (kV)	200
Cs (mm)	2.2
Detector	KODAK SO-163 FILM

3D Reconstruction

Method	HELICAL
Resolution (Å)	8.6
Software	UCSF Chimera,FREALIGN,SPIDER