3IYD
Three-dimensional EM structure of an intact activator-dependent transcription initiation complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B | DNA-directed RNA polymerase subunit alpha | polymer | 329 | 36558.7 | 2 | UniProt (P0A7Z4) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) In PDB | Escherichia coli | RNAP subunit alpha, Transcriptase subunit alpha, RNA polymerase subunit alpha |
| 2 | C | DNA-directed RNA polymerase subunit beta | polymer | 1342 | 150820.9 | 1 | UniProt (P0A8V2) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) In PDB | Escherichia coli | RNAP subunit beta, Transcriptase subunit beta, RNA polymerase subunit beta |
| 3 | D | DNA-directed RNA polymerase subunit beta | polymer | 1413 | 156195.6 | 1 | UniProt (P0A8T7) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) In PDB | RNAP subunit beta', Transcriptase subunit beta', RNA polymerase subunit beta' | |
| 4 | E | DNA-directed RNA polymerase subunit omega | polymer | 90 | 10118.4 | 1 | UniProt (P0A800) Pfam (PF01192) In PDB | Escherichia coli K-12 | RNAP omega subunit, Transcriptase subunit omega, RNA polymerase omega subunit |
| 5 | F | RNA polymerase sigma factor rpoD | polymer | 613 | 70326.2 | 1 | UniProt (P00579) Pfam (PF03979) Pfam (PF00140) Pfam (PF04546) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) In PDB | Escherichia coli K-12 | RNAP polymerase (sigma70 holoenzyme) |
| 6 | G, H | Catabolite gene activator | polymer | 209 | 23541.2 | 2 | UniProt (P0ACJ8) Pfam (PF00027) Pfam (PF13545) In PDB | Escherichia coli K-12 | cAMP receptor protein, cAMP regulatory protein, Catabolite activator protein |
| 7 | I | DNA (98-MER) | polymer | 98 | 30220.4 | 1 | Lac(ICAP)UP-UV5-BUBBLE | ||
| 8 | J | DNA (98-MER) | polymer | 98 | 30097.3 | 1 | |||
| 9 | G, H | ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE | non-polymer | 329.2 | 2 | Chemie (CMP) |
Sequence modifications
D: 1 - 1407 (UniProt: P0A8T7)
F: 9 - 613 (UniProt: P00579)
| PDB | External Database | Details |
|---|---|---|
| His 1408 | - | expression tag |
| His 1409 | - | expression tag |
| His 1410 | - | expression tag |
| His 1411 | - | expression tag |
| His 1412 | - | expression tag |
| His 1413 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Asn 149 | Asp 149 | conflict |
| His 571 | Tyr 571 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 10 |
| Total formula weight | 567978.6 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 658.4 | |
| All* | Total formula weight | 568637.0 |
