3IYD
Three-dimensional EM structure of an intact activator-dependent transcription initiation complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | DNA-directed RNA polymerase subunit alpha | polymer | 329 | 36558.7 | 2 | UniProt (P0A7Z4) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) | Escherichia coli | RNAP subunit alpha, Transcriptase subunit alpha, RNA polymerase subunit alpha |
| 2 | C (C) | DNA-directed RNA polymerase subunit beta | polymer | 1342 | 150820.9 | 1 | UniProt (P0A8V2) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) | Escherichia coli | RNAP subunit beta, Transcriptase subunit beta, RNA polymerase subunit beta |
| 3 | D (D) | DNA-directed RNA polymerase subunit beta | polymer | 1413 | 156195.6 | 1 | UniProt (P0A8T7) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) | RNAP subunit beta', Transcriptase subunit beta', RNA polymerase subunit beta' | |
| 4 | E (E) | DNA-directed RNA polymerase subunit omega | polymer | 90 | 10118.4 | 1 | UniProt (P0A800) Pfam (PF01192) | Escherichia coli K-12 | RNAP omega subunit, Transcriptase subunit omega, RNA polymerase omega subunit |
| 5 | F (F) | RNA polymerase sigma factor rpoD | polymer | 613 | 70326.2 | 1 | UniProt (P00579) Pfam (PF03979) Pfam (PF00140) Pfam (PF04546) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) | Escherichia coli K-12 | RNAP polymerase (sigma70 holoenzyme) |
| 6 | G, H (G, H) | Catabolite gene activator | polymer | 209 | 23541.2 | 2 | UniProt (P0ACJ8) Pfam (PF00027) Pfam (PF13545) | Escherichia coli K-12 | cAMP receptor protein, cAMP regulatory protein, Catabolite activator protein |
| 7 | I (I) | DNA (98-MER) | polymer | 98 | 30220.4 | 1 | Lac(ICAP)UP-UV5-BUBBLE | ||
| 8 | J (J) | DNA (98-MER) | polymer | 98 | 30097.3 | 1 | |||
| 9 | K, L (G, H) | ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE | non-polymer | 329.2 | 2 | Chemie (CMP) PubChem (176488666) PubChem (6076) PubChem (23902375) PubChem (25322948) PubChem (40467852) PubChem (44629928) PubChem (25322946) PubChem (56973690) PubChem (216878) PubChem (59909322) PubChem (59968988) PubChem (70878457) PubChem (59350541) PubChem (3080770) PubChem (3246347) PubChem (274) PubChem (6604194) PubChem (6603718) PubChem (6713792) PubChem (6714006) PubChem (124132380) PubChem (12071879) PubChem (12866365) PubChem (163043517) PubChem (129317551) PubChem (131841987) PubChem (134693155) PubChem (138107790) PubChem (163043518) |
Sequence modifications
D: 1 - 1407 (UniProt: P0A8T7)
F: 9 - 613 (UniProt: P00579)
| PDB | External Database | Details |
|---|---|---|
| His 1408 | - | expression tag |
| His 1409 | - | expression tag |
| His 1410 | - | expression tag |
| His 1411 | - | expression tag |
| His 1412 | - | expression tag |
| His 1413 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Asn 149 | Asp 149 | conflict |
| His 571 | Tyr 571 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 10 |
| Total formula weight | 567978.6 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 658.4 | |
| All* | Total formula weight | 568637.0 |
