3IYD
Three-dimensional EM structure of an intact activator-dependent transcription initiation complex
EM specimen
| Staining | Yes |
| Staining material | Uranyl Formate |
EM Data Collection
| Microscope | FEI TECNAI F20 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 120 |
| Cs (mm) | 2 |
| Detector | TVIPS TEMCAM-F415 (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 19.8 |
| Software | MODELLER,UCSF Chimera,Yup.scx,EMAN,SPIDER |
| Symmetry | C1 |
