3IT8

Crystal structure of TNF alpha complexed with a poxvirus MHC-related TNF binding protein

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, G, H...	Tumor necrosis factor	polymer	161	17955.4	6	UniProt (P01375) Pfam (PF00229) In PDB	Homo sapiens (human)	TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Cachectin, Tumor necrosis factor, membrane form, Tumor necrosis factor, soluble form
2	D, E, F, J, K...	2L protein	polymer	324	37325.6	6	UniProt (Q9DHW0) Pfam (PF21584) In PDB	Yaba-like disease virus (YLDV)
3	K, L, D, E, F...	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	18	Chemie (NAG)
4		water	water		18.0	96	Chemie (HOH)

Sequence modifications

A, B, C, G, H, I: 6 - 157 (UniProt: P01375)

PDB	External Database	Details
Met -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag
His 2	-	expression tag
His 3	-	expression tag
His 4	-	expression tag
His 5	-	expression tag
Leu 143	Asp 219	conflict

D, E, F, J, K, L: 1 - 316 (UniProt: Q9DHW0)

PDB	External Database	Details
Gly 317	-	expression tag
Ser 318	-	expression tag
His 319	-	expression tag
His 320	-	expression tag
His 321	-	expression tag
His 322	-	expression tag
His 323	-	expression tag
His 324	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	331685.7
Non-Polymers*	Number of molecules	18
	Total formula weight	3981.7
All*	Total formula weight	335667.5

*Water molecules are not included.