3ISM

Crystal structure of the EndoG/EndoGI complex: Mechanism of EndoG inhibition

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	CG8862	polymer	267	30587.3	2	UniProt (Q7JXB9) Pfam (PF01223) In PDB	Drosophila melanogaster (Fruit fly)	Endonuclease G, EndoG, LD35517p
2	C	CG4930	polymer	359	40643.1	1	UniProt (Q9V3V9) Pfam (PF08785) In PDB	Drosophila melanogaster (Fruit fly)	EndoGI, SD16985p
3	A, B	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
4	A	2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		122.1	1	Chemie (TRS)
5		water	water		18.0	328	Chemie (HOH)

Sequence modifications

A, B: 56 - 310 (UniProt: Q7JXB9)

PDB	External Database	Details
Met 55	-	initiating methionine
Ala 311	-	expression tag
Ala 312	-	expression tag
Ala 313	-	expression tag
Leu 314	-	expression tag
Glu 315	-	expression tag
His 316	-	expression tag
His 317	-	expression tag
His 318	-	expression tag
His 319	-	expression tag
His 320	-	expression tag
His 321	-	expression tag

C: 1 - 359 (UniProt: Q9V3V9)

PDB	External Database	Details
Ala 2	Ser 2	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	101817.7
Non-Polymers*	Number of molecules	3
	Total formula weight	170.8
All*	Total formula weight	101988.5

*Water molecules are not included.