3ISM
Crystal structure of the EndoG/EndoGI complex: Mechanism of EndoG inhibition
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-04-04 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0075 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 70.790, 109.150, 121.080 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.230 - 2.200 |
R-factor | 0.1864 |
Rwork | 0.184 |
R-free | 0.22308 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2o3b |
RMSD bond length | 0.010 |
RMSD bond angle | 1.150 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.230 | 2.300 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 46364 | |
<I/σ(I)> | 14.8 | 4.2 |
Completeness [%] | 95.9 | 90.5 |
Redundancy | 4.1 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 20%(w/v)PEG 2000 MME, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |