3INM
Crystal structure of human cytosolic NADP(+)-dependent isocitrate dehydrogenase R132H mutant in complex with NADPH, ALPHA-KETOGLUTARATE and CALCIUM(2+)
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B, C | Isocitrate dehydrogenase [NADP] cytoplasmic | polymer | 425 | 48135.7 | 3 | UniProt (O75874) Pfam (PF00180) In PDB | Homo sapiens (human) | IDH, Cytosolic NADP-isocitrate dehydrogenase, Oxalosuccinate decarboxylase, NADP(+)-specific ICDH, IDP |
2 | A, B, C | NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE | non-polymer | 745.4 | 3 | Chemie (NDP) | |||
3 | A, B, C | 2-OXOGLUTARIC ACID | non-polymer | 146.1 | 3 | Chemie (AKG) | |||
4 | A, B, C | CALCIUM ION | non-polymer | 40.1 | 3 | Chemie (CA) | |||
5 | A, B, C | SODIUM ION | non-polymer | 23.0 | 3 | Chemie (NA) | |||
6 | A, C | GLYCEROL | non-polymer | 92.1 | 3 | Chemie (GOL) | |||
7 | water | water | 18.0 | 301 | Chemie (HOH) |
Sequence modifications
A, B, C: 1 - 414 (UniProt: O75874)
PDB | External Database | Details |
---|---|---|
His 132 | Arg 132 | engineered mutation |
Ser 415 | - | expression tag |
Leu 416 | - | expression tag |
Glu 417 | - | expression tag |
His 418 | - | expression tag |
His 419 | - | expression tag |
His 420 | - | expression tag |
His 421 | - | expression tag |
His 422 | - | expression tag |
His 423 | - | expression tag |
His 424 | - | expression tag |
His 425 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 3 |
Total formula weight | 144407.1 | |
Non-Polymers* | Number of molecules | 15 |
Total formula weight | 3140.0 | |
All* | Total formula weight | 147547.1 |