3IF9

Crystal structure of Glycine Oxidase G51S/A54R/H244A mutant in complex with inhibitor glycolate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Glycine oxidase	polymer	382	42699.8	4	UniProt (O31616) Pfam (PF01266)	Bacillus subtilis
2	E, G, I, K (A, B, C, D)	GLYCOLIC ACID	non-polymer		76.1	4	Chemie (GOA)
3	F, H, J, L (A, B, C, D)	FLAVIN-ADENINE DINUCLEOTIDE	non-polymer		785.5	4	Chemie (FAD)
4	M, N, O, P (A, B, C, D)	water	water		18.0	156	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 369 (UniProt: O31616)

PDB	External Database	Details
Met -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
Met -5	-	expression tag
Ala -4	-	expression tag
Arg -3	-	expression tag
Ile -2	-	expression tag
Arg -1	-	expression tag
Ala 0	-	expression tag
Ser 51	Gly 51	engineered mutation
Arg 54	Ala 54	engineered mutation
Ala 244	His 244	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	170799.3
Non-Polymers*	Number of molecules	8
	Total formula weight	3446.4
All*	Total formula weight	174245.7

*Water molecules are not included.