3IAX

The crystal structure of the TolB box of Colicin A in complex with TolB reveals important differences in the recruitment of the common TolB translocation portal used by group A colicins

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Protein tolB	polymer	438	47065.4	1	UniProt (P0A855) Pfam (PF04052) Pfam (PF07676) In PDB	Escherichia coli
2	B	Colicin-A	polymer	115	11969.9	1	UniProt (P04480) Pfam (PF22348) In PDB	Citrobacter freundii
3	A	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
4	A	CALCIUM ION	non-polymer		40.1	1	Chemie (CA)
5	A	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
6		water	water		18.0	62	Chemie (HOH)

Sequence modifications

A: 1 - 430 (UniProt: P0A855)

PDB	External Database	Details
Leu 431	-	expression tag
Glu 432	-	expression tag
His 433	-	expression tag
His 434	-	expression tag
His 435	-	expression tag
His 436	-	expression tag
His 437	-	expression tag
His 438	-	expression tag

B: 1 - 107 (UniProt: P04480)

PDB	External Database	Details
Leu 108	-	expression tag
Glu 109	-	expression tag
His 110	-	expression tag
His 111	-	expression tag
His 112	-	expression tag
His 113	-	expression tag
His 114	-	expression tag
His 115	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	59035.3
Non-Polymers*	Number of molecules	3
	Total formula weight	155.2
All*	Total formula weight	59190.5

*Water molecules are not included.