3IAX
The crystal structure of the TolB box of Colicin A in complex with TolB reveals important differences in the recruitment of the common TolB translocation portal used by group A colicins
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-05-18 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.955, 40.174, 80.868 |
| Unit cell angles | 90.00, 97.18, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.600 |
| R-factor | 0.19316 |
| Rwork | 0.190 |
| R-free | 0.25685 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1c5k |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.774 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.670 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.095 | 0.347 |
| Number of reflections | 12220 | |
| <I/σ(I)> | 7.7 | 3.7 |
| Completeness [%] | 97.9 | 96.6 |
| Redundancy | 3.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 20% PEG 10000, 0.1 M Hepes, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
