3IAQ
E. coli (lacz) beta-galactosidase (E416V)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | Beta-galactosidase | polymer | 1023 | 116476.3 | 4 | UniProt (B8LFD6) Pfam (PF02837) Pfam (PF00703) Pfam (PF02836) Pfam (PF16353) Pfam (PF02929) UniProt (by SIFTS) (P00722) | Escherichia coli K-12 | |
| 2 | E, F, G, H, JA... (A, B, C, D) | MAGNESIUM ION | non-polymer | 24.3 | 13 | Chemie (MG) | |||
| 3 | AD, BD, I, J, K... (D, A, B, C) | SODIUM ION | non-polymer | 23.0 | 14 | Chemie (NA) | |||
| 4 | AA, AB, AC, BA, BB... (A, B, C, D) | DIMETHYL SULFOXIDE | non-polymer | 78.1 | 91 | Chemie (DMS) | |||
| 5 | HA, MB, VD, WC (A, B, D, C) | 2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL | non-polymer | 209.2 | 4 | Chemie (BTB) | |||
| 6 | WD, XD, YD, ZD (A, B, C, D) | water | water | 18.0 | 2393 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 9 - 1023 (UniProt: B8LFD6)
| PDB | External Database | Details |
|---|---|---|
| Gly 1 | - | expression tag |
| Ser 2 | - | expression tag |
| His 3 | - | expression tag |
| Met 4 | - | expression tag |
| Leu 5 | - | expression tag |
| Glu 6 | - | expression tag |
| Asp 7 | - | expression tag |
| Pro 8 | - | expression tag |
| Val 416 | Glu 417 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 465905.1 | |
| Non-Polymers* | Number of molecules | 122 |
| Total formula weight | 8584.9 | |
| All* | Total formula weight | 474490.0 |
