3IAQ
E. coli (lacz) beta-galactosidase (E416V)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-08-01 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 149.380, 168.300, 200.730 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 83.330 - 2.700 |
| R-factor | 0.1848 |
| Rwork | 0.184 |
| R-free | 0.25312 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dp0 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.950 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 83.300 | |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 291981 | |
| Completeness [%] | 100.0 | 75.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 288 | PEG8000, NaCl, MgCl2, DTT, Bis-Tris, pH6.5, HANGING DROP AT 288K, VAPOR DIFFUSION, HANGING DROP |
