3HST

N-Terminal RNASE H domain of rv2228c from mycobacterium tuberculosis as a fusion protein with maltose binding protein

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	Maltose-binding periplasmic protein	polymer	387	42526.8	2	UniProt (P0AEX9) Pfam (PF01547)	Escherichia coli K-12	MMBP, Maltodextrin-binding protein
2	B, D (B, D)	Protein Rv2228c/MT2287	polymer	141	15058.9	2	UniProt (P64955) Pfam (PF13456) UniProt (by SIFTS) (P9WLH5)	Mycobacterium tuberculosis
3	E, F (E, F)	alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose	branched		504.4	2	In PDB BIRD (PRD_900009) GlyTouCan (G96370VA)		alpha-maltotriose
4	G, H, J (A, C)	1,2-ETHANEDIOL	non-polymer		62.1	3	Chemie (EDO)
5	I (C)	D(-)-TARTARIC ACID	non-polymer		150.1	1	Chemie (TAR)
6	K, L, M, N (A, B, C, D)	water	water		18.0	305	Chemie (HOH)

Sequence modifications

A, C: 9 - 374 (UniProt: P0AEX9)

PDB	External Database	Details
Met 8	-	expression tag
Asn 375	-	expression tag
Ser 376	-	expression tag
Ser 377	-	expression tag
Ser 378	-	expression tag
Asn 379	-	expression tag
Asn 380	-	expression tag
Asn 381	-	expression tag
Asn 382	-	expression tag
Asn 383	-	expression tag
Asn 384	-	expression tag
Asn 385	-	expression tag
Asn 386	-	expression tag
Asn 387	-	expression tag
Asn 388	-	expression tag
Leu 389	-	expression tag
Gly 390	-	expression tag
Ile 391	-	expression tag
Glu 392	-	expression tag
Gly 393	-	expression tag
Arg 394	-	expression tag

B, D: 1 - 140 (UniProt: P64955)

PDB	External Database	Details
Ser 0	-	expression tag
Val 1	1	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	115171.4
Branched	Number of molecules	2
	Total formula weight	1008.9
Non-Polymers*	Number of molecules	4
	Total formula weight	336.3
All*	Total formula weight	116516.6

*Water molecules are not included.