3HST

N-Terminal RNASE H domain of rv2228c from mycobacterium tuberculosis as a fusion protein with maltose binding protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	113
Detector technology	CCD
Collection date	2007-02-27
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.98397
Spacegroup name	P 1 21 1
Unit cell lengths	73.632, 101.383, 76.091
Unit cell angles	90.00, 109.01, 90.00

Refinement procedure

Resolution	41.450 - 2.250
R-factor	0.18549
Rwork	0.183
R-free	0.23854
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.022
RMSD bond angle	1.901
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.3.0037)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.450	2.370
High resolution limit [Å]	2.250	2.250
Rmerge		0.121
Number of reflections	50220
<I/σ(I)>		11.5
Completeness [%]	100.0	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	20% PEG2000, 0.2M Ammounium tartrate, pH pH7.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K