3HP6
Crystal structure of fragment DNA polymerase I from Bacillus stearothermophilus F710Y mutant bound to G:T mismatch
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, D) | DNA polymerase I, large fragment | polymer | 580 | 66221.0 | 2 | Pfam (PF22619) Pfam (PF00476) UniProt (by SIFTS) (Q5KWC1) | Geobacillus stearothermophilus | |
| 2 | C, E (B, E) | 5'-D(*CP*GP*AP*TP*CP*AP*CP*GP*(DDG))-3' | polymer | 9 | 2724.8 | 2 | |||
| 3 | D, F (C, F) | 5'-D(*AP*CP*GP*CP*CP*GP*TP*GP*AP*TP*CP*G)-3' | polymer | 12 | 3663.4 | 2 | |||
| 4 | G, H (G, H) | beta-D-fructofuranose-(2-1)-alpha-D-glucopyranose | branched | 342.3 | 2 | In PDB BIRD (PRD_900003) | sucrose | ||
| 5 | I, L, O (A, D) | SULFATE ION | non-polymer | 96.1 | 3 | Chemie (SO4) | |||
| 6 | J, M (A, D) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 7 | K, N (A, D) | 2',3'-DIDEOXY-THYMIDINE-5'-TRIPHOSPHATE | non-polymer | 466.2 | 2 | Chemie (D3T) | |||
| 8 | P, Q, R, S, T... (A, D, B, C, E...) | water | water | 18.0 | 869 | Chemie (HOH) |
Sequence modifications
A, D: 297 - 876 (PDB: 3HP6)
| PDB | External Database | Details |
|---|---|---|
| Ala 598 | Asp 598 | engineered mutation |
| Tyr 710 | Phe 710 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 145218.3 | |
| Branched | Number of molecules | 2 |
| Total formula weight | 684.6 | |
| Non-Polymers* | Number of molecules | 7 |
| Total formula weight | 1269.1 | |
| All* | Total formula weight | 147172.1 |
