3HP6

Crystal structure of fragment DNA polymerase I from Bacillus stearothermophilus F710Y mutant bound to G:T mismatch

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D	DNA polymerase I, large fragment	polymer	580	66221.0	2	Pfam (PF22619) Pfam (PF00476) UniProt (by SIFTS) (Q5KWC1) In PDB	Geobacillus stearothermophilus
2	B, E	5'-D(*CP*GP*AP*TP*CP*AP*CP*GP*(DDG))-3'	polymer	9	2724.8	2
3	C, F	5'-D(*AP*CP*GP*CP*CP*GP*TP*GP*AP*TP*CP*G)-3'	polymer	12	3663.4	2
4	G, H	beta-D-fructofuranose-(2-1)-alpha-D-glucopyranose	branched		342.3	2	In PDB BIRD (PRD_900003)		sucrose
5	A, D	SULFATE ION	non-polymer		96.1	3	Chemie (SO4)
6	A, D	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
7	A, D	2',3'-DIDEOXY-THYMIDINE-5'-TRIPHOSPHATE	non-polymer		466.2	2	Chemie (D3T)
8		water	water		18.0	869	Chemie (HOH)

Sequence modifications

A, D: 297 - 876 (PDB: 3HP6)

PDB	External Database	Details
Ala 598	Asp 598	engineered mutation
Tyr 710	Phe 710	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	145218.3
Branched	Number of molecules	2
	Total formula weight	684.6
Non-Polymers*	Number of molecules	7
	Total formula weight	1269.1
All*	Total formula weight	147172.1

*Water molecules are not included.