3HP6
Crystal structure of fragment DNA polymerase I from Bacillus stearothermophilus F710Y mutant bound to G:T mismatch
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-04-04 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 93.170, 108.710, 152.210 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.840 - 1.810 |
R-factor | 0.21295 |
Rwork | 0.211 |
R-free | 0.24723 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2hvi |
RMSD bond length | 0.012 |
RMSD bond angle | 1.455 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.840 | 1.860 |
High resolution limit [Å] | 1.810 | 1.810 |
Rmerge | 0.058 | 0.592 |
Number of reflections | 139378 | |
<I/σ(I)> | 2.61 | |
Completeness [%] | 98.9 | 98.1 |
Redundancy | 5 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 290 | 50% Saturated ammonium sulfate, 0.1M MES, 2.5% v/v MPD, 10mM Magnesium sulfate, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | ammonium sulfate | ||
2 | 1 | 1 | MES | ||
3 | 1 | 1 | MPD | ||
4 | 1 | 1 | Magnesium sulfate | ||
5 | 1 | 2 | ammonium sulfate | ||
6 | 1 | 2 | MES | ||
7 | 1 | 2 | MPD | ||
8 | 1 | 2 | Magnesium sulfate |