3HOW
Complete RNA polymerase II elongation complex III with a T-U mismatch and a frayed RNA 3'-uridine
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | DNA-directed RNA polymerase II subunit RPB1 | polymer | 1733 | 191821.6 | 1 | UniProt (P04050) Pfam (PF04997) Pfam (PF05001) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) Pfam (PF04992) Pfam (PF04990) | Saccharomyces cerevisiae (Baker's yeast) | RNA polymerase II subunit B1, RNA polymerase II subunit 1, DNA-directed RNA polymerase III largest subunit, RNA polymerase II subunit B220 |
| 2 | B (B) | DNA-directed RNA polymerase II subunit RPB2 | polymer | 1224 | 138937.3 | 1 | UniProt (P08518) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF04566) Pfam (PF04567) Pfam (PF00562) Pfam (PF04560) | Saccharomyces cerevisiae (Baker's yeast) | RNA polymerase II subunit 2, DNA-directed RNA polymerase II 140 kDa polypeptide, B150 |
| 3 | C (C) | DNA-directed RNA polymerase II subunit RPB3 | polymer | 347 | 38677.9 | 1 | UniProt (P16370) Pfam (PF01193) Pfam (PF01000) | Saccharomyces cerevisiae (Baker's yeast) | RNA polymerase II subunit B3, RNA polymerase II subunit 3, DNA-directed RNA polymerase II 45 kDa polypeptide, B44.5 |
| 4 | D (D) | DNA-directed RNA polymerase II subunit RPB4 | polymer | 221 | 25451.2 | 1 | UniProt (P20433) Pfam (PF03874) | Saccharomyces cerevisiae (Baker's yeast) | RNA polymerase II subunit B4, DNA-directed RNA polymerase II 32 kDa polypeptide, B32 |
| 5 | E (E) | DNA-directed RNA polymerases I, II, and III subunit RPABC1 | polymer | 215 | 25117.1 | 1 | UniProt (P20434) Pfam (PF03871) Pfam (PF01191) | Saccharomyces cerevisiae (Baker's yeast) | RNA polymerases I, II, and III subunit ABC1, DNA-directed RNA polymerases I, II, and III 27 kDa polypeptide, ABC27 |
| 6 | F (F) | DNA-directed RNA polymerases I, II, and III subunit RPABC2 | polymer | 155 | 17931.8 | 1 | UniProt (P20435) Pfam (PF01192) | Saccharomyces cerevisiae (Baker's yeast) | RNA polymerases I, II, and III subunit ABC2, DNA-directed RNA polymerases I, II, and III 23 kDa polypeptide, ABC23 |
| 7 | G (G) | DNA-directed RNA polymerase II subunit RPB7 | polymer | 171 | 19081.1 | 1 | UniProt (P34087) Pfam (PF03876) Pfam (PF00575) | Saccharomyces cerevisiae (Baker's yeast) | RNA polymerase II subunit B7, B16 |
| 8 | H (H) | DNA-directed RNA polymerases I, II, and III subunit RPABC3 | polymer | 146 | 16525.4 | 1 | UniProt (P20436) Pfam (PF03870) | Saccharomyces cerevisiae (Baker's yeast) | RNA polymerases I, II, and III subunit ABC3, DNA-directed RNA polymerases I, II, and III 14.5 kDa polypeptide, ABC14.5, ABC14.4 |
| 9 | I (I) | DNA-directed RNA polymerase II subunit RPB9 | polymer | 122 | 14308.2 | 1 | UniProt (P27999) Pfam (PF02150) Pfam (PF01096) | Saccharomyces cerevisiae (Baker's yeast) | RNA polymerase II subunit B9, DNA-directed RNA polymerase II subunit 9, DNA-directed RNA polymerase II 14.2 kDa polypeptide, B12.6 |
| 10 | J (J) | DNA-directed RNA polymerases I, II, and III subunit RPABC5 | polymer | 70 | 8290.7 | 1 | UniProt (P22139) Pfam (PF01194) | Saccharomyces cerevisiae (Baker's yeast) | RNA polymerases I, II, and III subunit ABC5, DNA-directed RNA polymerases I, II, and III 8.3 kDa polypeptide, ABC10-beta, ABC8 |
| 11 | K (K) | DNA-directed RNA polymerase II subunit RPB11 | polymer | 120 | 13633.5 | 1 | UniProt (P38902) Pfam (PF13656) | Saccharomyces cerevisiae (Baker's yeast) | RNA polymerase II subunit B11, DNA-directed RNA polymerase II 13.6 kDa polypeptide, B13.6 |
| 12 | L (L) | DNA-directed RNA polymerases I, II, and III subunit RPABC4 | polymer | 70 | 7730.0 | 1 | UniProt (P40422) Pfam (PF03604) | Saccharomyces cerevisiae (Baker's yeast) | RNA polymerases I, II, and III subunit ABC4, ABC10-alpha |
| 13 | M (2) | 5'-D(*AP*CP*TP*AP*CP*TP*TP*GP*AP*GP*CP*T)-3' | polymer | 12 | 3637.4 | 1 | NONTEMPLATE DNA | ||
| 14 | N (1) | 5'-D(*AP*GP*CP*TP*C*AP*AP*GP*TP*AP*GP*TP*TP*AP*TP*GP*CP*CP*(BRU)P*GP*GP*TP*CP*AP*TP*T)-3' | polymer | 26 | 8058.0 | 1 | TEMPLATE DNA | ||
| 15 | O (3) | 5'-R(*UP*GP*CP*AP*UP*UP*U*CP*AP*AP*CP*CP*AP*GP*GP*CP*UP*U)-3' | polymer | 18 | 5670.4 | 1 | PRODUCT RNA | ||
| 16 | P, Q, S, T, U... (A, B, C, I, J...) | ZINC ION | non-polymer | 65.4 | 8 | Chemie (ZN) | |||
| 17 | R (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) |
Sequence modifications
C: 2 - 318 (UniProt: P16370)
| PDB | External Database | Details |
|---|---|---|
| Met -28 | - | expression tag |
| Gly -27 | - | expression tag |
| Ser -26 | - | expression tag |
| His -25 | - | expression tag |
| His -24 | - | expression tag |
| His -23 | - | expression tag |
| His -22 | - | expression tag |
| His -21 | - | expression tag |
| His -20 | - | expression tag |
| Ser -19 | - | expression tag |
| Asn -18 | - | expression tag |
| Ser -17 | - | expression tag |
| Gly -16 | - | expression tag |
| Leu -15 | - | expression tag |
| Asn -14 | - | expression tag |
| Asp -13 | - | expression tag |
| Ile -12 | - | expression tag |
| Phe -11 | - | expression tag |
| Glu -10 | - | expression tag |
| Ala -9 | - | expression tag |
| Gln -8 | - | expression tag |
| Lys -7 | - | expression tag |
| Ile -6 | - | expression tag |
| Glu -5 | - | expression tag |
| Trp -4 | - | expression tag |
| His -3 | - | expression tag |
| Glu -2 | - | expression tag |
| Asp -1 | - | expression tag |
| Thr 0 | - | expression tag |
| Gly 1 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 15 |
| Total formula weight | 534871.5 | |
| Non-Polymers* | Number of molecules | 9 |
| Total formula weight | 547.6 | |
| All* | Total formula weight | 535419.1 |
