3HK2
Crystal structure of T. thermophilus Argonaute N478 mutant protein complexed with DNA guide strand and 19-nt RNA target strand
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | Argonaute | polymer | 685 | 76727.8 | 2 | UniProt (Q746M7) Pfam (PF22472) Pfam (PF18309) Pfam (PF22474) In PDB | Thermus thermophilus | |
2 | C, E | 5'-D(P*TP*GP*AP*GP*GP*TP*AP*GP*TP*AP*GP*GP*TP*TP*GP*TP*AP*TP*AP*GP*T)-3' | polymer | 21 | 6588.3 | 2 | |||
3 | F, D | 5'-R(*UP*AP*UP*AP*CP*AP*AP*CP*CP*UP*AP*CP*UP*AP*CP*CP*UP*CP*G)-3' | polymer | 19 | 5942.6 | 2 | |||
4 | A, B, C, E | MAGNESIUM ION | non-polymer | 24.3 | 4 | Chemie (MG) | |||
5 | water | water | 18.0 | 14 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 685 (UniProt: Q746M7)
PDB | External Database | Details |
---|---|---|
Asn 478 | Asp 478 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 6 |
Total formula weight | 178517.2 | |
Non-Polymers* | Number of molecules | 4 |
Total formula weight | 97.2 | |
All* | Total formula weight | 178614.4 |