3HK2

Crystal structure of T. thermophilus Argonaute N478 mutant protein complexed with DNA guide strand and 19-nt RNA target strand

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Argonaute	polymer	685	76727.8	2	UniProt (Q746M7) Pfam (PF22472) Pfam (PF18309) Pfam (PF22474)	Thermus thermophilus
2	C, D (C, E)	5'-D(P*TP*GP*AP*GP*GP*TP*AP*GP*TP*AP*GP*GP*TP*TP*GP*TP*AP*TP*AP*GP*T)-3'	polymer	21	6588.3	2
3	E, F (F, D)	5'-R(*UP*AP*UP*AP*CP*AP*AP*CP*CP*UP*AP*CP*UP*AP*CP*CP*UP*CP*G)-3'	polymer	19	5942.6	2
4	G, H, I, J (A, B, C, E)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
5	K, L, M (A, B, E)	water	water		18.0	14	Chemie (HOH)

Sequence modifications

A, B: 1 - 685 (UniProt: Q746M7)

PDB	External Database	Details
Asn 478	Asp 478	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	178517.2
Non-Polymers*	Number of molecules	4
	Total formula weight	97.2
All*	Total formula weight	178614.4

*Water molecules are not included.