3HK2

Crystal structure of T. thermophilus Argonaute N478 mutant protein complexed with DNA guide strand and 19-nt RNA target strand

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2009-03-17
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97
Spacegroup name	P 21 21 21
Unit cell lengths	111.193, 110.884, 174.339
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.781 - 2.800
R-factor	0.207
Rwork	0.205
R-free	0.24100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3dlh
RMSD bond length	0.007
RMSD bond angle	1.242
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.056	0.487
Number of reflections	53789
<I/σ(I)>	30.6	2.1
Completeness [%]	99.9	99.9
Redundancy	6.6	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	308	1.0 M succinic acid, 0.1 M Hepes, pH 7.0, 1% (w/v) polyethylene glycol monomethyl ether 2,000, VAPOR DIFFUSION, HANGING DROP, temperature 308 K