3H85

Molecular basis for the association of PIPKI gamma-p90 with the clathrin adaptor AP-2

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	AP-2 complex subunit mu-1	polymer	299	34100.8	1	UniProt (P84092) Pfam (PF00928) In PDB	Rattus norvegicus (Rat)	Mu2-adaptin, AP-2 mu-2 chain, Plasma membrane adaptor AP-2 50 kDa protein, Clathrin assembly protein complex 2 medium chain, Clathrin coat assembly protein AP50, Clathrin coat-associated protein AP50
2	P	Phosphatidylinositol-4-phosphate 5-kinase type-1 gamma	polymer	8	989.1	1	UniProt (O60331) In PDB		Phosphatidylinositol-4-phosphate 5-kinase type I gamma, PtdIns(4)P-5-kinase gamma, PtdInsPKIgamma, PIP5KIgamma
3	A	NICKEL (II) ION	non-polymer		58.7	1	Chemie (NI)
4		water	water		18.0	31	Chemie (HOH)

Sequence modifications

A: 158 - 435 (UniProt: P84092)

PDB	External Database	Details
Met 137	-	expression tag
Gly 138	-	expression tag
Ser 139	-	expression tag
Ser 140	-	expression tag
His 141	-	expression tag
His 142	-	expression tag
His 143	-	expression tag
His 144	-	expression tag
His 145	-	expression tag
His 146	-	expression tag
Ser 147	-	expression tag
Ser 148	-	expression tag
Gly 149	-	expression tag
Leu 150	-	expression tag
Val 151	-	expression tag
Pro 152	-	expression tag
Arg 153	-	expression tag
Gly 154	-	expression tag
Ser 155	-	expression tag
His 156	-	expression tag
Met 157	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	35089.9
Non-Polymers*	Number of molecules	1
	Total formula weight	58.7
All*	Total formula weight	35148.6

*Water molecules are not included.