3H85
Molecular basis for the association of PIPKI gamma-p90 with the clathrin adaptor AP-2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-12-20 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.91841 |
Spacegroup name | P 64 |
Unit cell lengths | 125.301, 125.301, 74.546 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 41.010 - 2.600 |
R-factor | 0.233 |
Rwork | 0.231 |
R-free | 0.26300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1bw8 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.151 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.690 |
High resolution limit [Å] | 2.600 | 5.600 | 2.600 |
Rmerge | 0.069 | 0.045 | 0.484 |
Number of reflections | 19090 | ||
<I/σ(I)> | 15.965 | ||
Completeness [%] | 92.6 | 99.4 | 49 |
Redundancy | 3.5 | 3.6 | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 1.4M sodium formate, 50mM NiCl, 0.1M Na-acetate, pH6.0, VAPOR DIFFUSION, HANGING DROP, temperature 291.0K |