3H44
Crystal Structure of Insulin Degrading Enzyme in Complex with macrophage inflammatory protein 1 alpha
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Insulin-degrading enzyme | polymer | 990 | 114560.6 | 2 | UniProt (P14735) Pfam (PF00675) Pfam (PF05193) Pfam (PF16187) | Homo sapiens (human) | Insulin protease, Insulinase, Insulysin |
| 2 | C, D (C, D) | C-C motif chemokine 3 | polymer | 70 | 7793.7 | 2 | UniProt (P10147) Pfam (PF00048) | Homo sapiens (human) | Small-inducible cytokine A3, Macrophage inflammatory protein 1-alpha, MIP-1-alpha, Tonsillar lymphocyte LD78 alpha protein, G0/G1 switch regulatory protein 19-1, G0S19-1 protein, SIS-beta, PAT 464.1, LD78-alpha |
| 3 | E, F, G, I, J... (A, B) | 1,4-DIETHYLENE DIOXIDE | non-polymer | 88.1 | 6 | Chemie (DIO) | |||
| 4 | H, L (B, C) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
| 5 | M, N, O, P (A, B, C, D) | water | water | 18.0 | 133 | Chemie (HOH) |
Sequence modifications
A, B: 42 - 1019 (UniProt: P14735)
| PDB | External Database | Details |
|---|---|---|
| Met 30 | - | expression tag |
| His 31 | - | expression tag |
| His 32 | - | expression tag |
| His 33 | - | expression tag |
| His 34 | - | expression tag |
| His 35 | - | expression tag |
| His 36 | - | expression tag |
| Ala 37 | - | expression tag |
| Ala 38 | - | expression tag |
| Gly 39 | - | expression tag |
| Ile 40 | - | expression tag |
| Pro 41 | - | expression tag |
| Leu 110 | Cys 110 | engineered mutation |
| Gln 111 | Glu 111 | engineered mutation |
| Ser 171 | Cys 171 | engineered mutation |
| Ala 178 | Cys 178 | engineered mutation |
| Val 257 | Cys 257 | engineered mutation |
| Leu 414 | Cys 414 | engineered mutation |
| Asn 573 | Cys 573 | engineered mutation |
| Ser 590 | Cys 590 | engineered mutation |
| Ser 789 | Cys 789 | engineered mutation |
| Ala 812 | Cys 812 | engineered mutation |
| Ala 819 | Cys 819 | engineered mutation |
| Ser 904 | Cys 904 | engineered mutation |
| Asn 966 | Cys 966 | engineered mutation |
| Ala 974 | Cys 974 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 244708.5 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 659.4 | |
| All* | Total formula weight | 245367.9 |
