3GVK
Crystal structure of endo-neuraminidase NF mutant
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C | Endo-N-acetylneuraminidase | polymer | 670 | 74475.4 | 3 | UniProt (Q858B1) Pfam (PF12218) Pfam (PF12217) Pfam (PF12195) Pfam (PF12219) UniProt (by SIFTS) (Q04830) In PDB | Enterobacteria phage K1F (Bacteriophage K1F) | Endo-alpha-sialidase, Gp17 protein |
| 2 | D, E, G, H, J... | N-acetyl-alpha-neuraminic acid-(2-8)-N-acetyl-alpha-neuraminic acid-(2-8)-N-acetyl-beta-neuraminic acid | branched | 891.8 | 6 | In PDB GlyTouCan (G23567MS) | |||
| 3 | F, I, L | N-acetyl-alpha-neuraminic acid-(2-8)-N-acetyl-beta-neuraminic acid | branched | 600.5 | 3 | In PDB GlyTouCan (G50645ZL) | |||
| 4 | A, B, C | SODIUM ION | non-polymer | 23.0 | 3 | Chemie (NA) | |||
| 5 | water | water | 18.0 | 2130 | Chemie (HOH) |
Sequence modifications
A, B, C: 246 - 910 (UniProt: Q858B1)
| PDB | External Database | Details |
|---|---|---|
| Val 241 | - | expression tag |
| Pro 242 | - | expression tag |
| Arg 243 | - | expression tag |
| Gly 244 | - | expression tag |
| Ser 245 | - | expression tag |
| Ala 350 | His 350 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 223426.2 | |
| Branched | Number of molecules | 9 |
| Total formula weight | 7152.3 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 69.0 | |
| All* | Total formula weight | 230647.4 |
