3GVK
Crystal structure of endo-neuraminidase NF mutant
Functional Information from GO Data
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 6 |
| Details | ACT_SITE: ACT_SITE => ECO:0000269|PubMed:15608653, ECO:0000269|PubMed:20096705 |
| Chain | Residue | Details |
| A | GLU581 | |
| A | ARG647 | |
| B | GLU581 | |
| B | ARG647 | |
| C | GLU581 | |
| C | ARG647 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 3 |
| Details | ACT_SITE: ACT_SITE => ECO:0000269|PubMed:15608653 |
| Chain | Residue | Details |
| A | ARG596 | |
| B | ARG596 | |
| C | ARG596 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 18 |
| Details | SITE: Binding to sialic acid => ECO:0000269|PubMed:20096705 |
| Chain | Residue | Details |
| A | HIS542 | |
| B | ASP578 | |
| B | THR598 | |
| B | ARG599 | |
| C | HIS542 | |
| C | ASP545 | |
| C | ARG549 | |
| C | ASP578 | |
| C | THR598 | |
| C | ARG599 | |
| A | ASP545 | |
| A | ARG549 | |
| A | ASP578 | |
| A | THR598 | |
| A | ARG599 | |
| B | HIS542 | |
| B | ASP545 | |
| B | ARG549 |
Catalytic Information from CSA
| site_id | MCSA1 |
| Number of Residues | 5 |
| Details | M-CSA 829 |
| Chain | Residue | Details |
| A | TYR325 | promote heterolysis, proton acceptor, proton donor |
| A | ALA350 | promote heterolysis, proton acceptor, proton donor, proton relay |
| A | GLU581 | activator, electrostatic stabiliser, increase nucleophilicity, promote heterolysis, proton acceptor, proton donor |
| A | ARG596 | electrostatic stabiliser, increase acidity |
| A | ARG647 | electrostatic stabiliser, increase acidity |
| site_id | MCSA2 |
| Number of Residues | 5 |
| Details | M-CSA 829 |
| Chain | Residue | Details |
| B | TYR325 | promote heterolysis, proton acceptor, proton donor |
| B | ALA350 | promote heterolysis, proton acceptor, proton donor, proton relay |
| B | GLU581 | activator, electrostatic stabiliser, increase nucleophilicity, promote heterolysis, proton acceptor, proton donor |
| B | ARG596 | electrostatic stabiliser, increase acidity |
| B | ARG647 | electrostatic stabiliser, increase acidity |
| site_id | MCSA3 |
| Number of Residues | 5 |
| Details | M-CSA 829 |
| Chain | Residue | Details |
| C | TYR325 | promote heterolysis, proton acceptor, proton donor |
| C | ALA350 | promote heterolysis, proton acceptor, proton donor, proton relay |
| C | GLU581 | activator, electrostatic stabiliser, increase nucleophilicity, promote heterolysis, proton acceptor, proton donor |
| C | ARG596 | electrostatic stabiliser, increase acidity |
| C | ARG647 | electrostatic stabiliser, increase acidity |
