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T4 lysozyme M102E/L99A mutant with buried charge in apolar cavity--Apo structure
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Lysozyme | polymer | 164 | 18785.7 | 1 | UniProt (P00720) Pfam (PF00959) | Enterobacteria phage T4 | Lysis protein, Muramidase, Endolysin |
| 2 | B (A) | BETA-MERCAPTOETHANOL | non-polymer | 78.1 | 1 | Chemie (BME) | |||
| 3 | C (A) | CARBONATE ION | non-polymer | 60.0 | 1 | Chemie (CO3) | |||
| 4 | D (A) | TRIS(HYDROXYETHYL)AMINOMETHANE | non-polymer | 163.2 | 1 | Chemie (TAM) | |||
| 5 | E (A) | SULFATE ION | non-polymer | 96.1 | 1 | Chemie (SO4) | |||
| 6 | F (A) | water | water | 18.0 | 206 | Chemie (HOH) |
Sequence modifications
A: 1 - 164 (UniProt: P00720)
| PDB | External Database | Details |
|---|---|---|
| Cys 21 | Thr 21 | engineered mutation |
| Asp 38 | Ser 38 | engineered mutation |
| Ala 99 | Leu 99 | engineered mutation |
| Glu 102 | Met 102 | engineered mutation |
| Val 108 | Glu 108 | engineered mutation |
| Val 117 | Ser 117 | engineered mutation |
| Cys 142 | Thr 142 | engineered mutation |
| Asp 144 | Asn 144 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 18785.7 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 397.4 | |
| All* | Total formula weight | 19183.1 |
