3GKL
Following evolutionary paths to high affinity and selectivity protein-protein interactions using Colicin7 and Immunity proteins
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | Colicin-E9 immunity protein | polymer | 141 | 16230.4 | 2 | UniProt (Q47112) Pfam (PF21431) In PDB | Escherichia coli | |
2 | C, D | Colicin-E7 | polymer | 86 | 9505.3 | 2 | UniProt (P13479) Pfam (PF01320) In PDB | Escherichia coli | ImmE9, Microcin-E9 immunity protein |
3 | A, B | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
4 | water | water | 18.0 | 10 | Chemie (HOH) |
Sequence modifications
A, B: 446 - 576 (UniProt: Q47112)
C, D: 1001 - 1086 (UniProt: P13479)
PDB | External Database | Details |
---|---|---|
Met 436 | - | expression tag |
His 437 | - | expression tag |
His 438 | - | expression tag |
His 439 | - | expression tag |
His 440 | - | expression tag |
His 441 | - | expression tag |
His 442 | - | expression tag |
Ser 443 | - | expression tag |
Met 444 | - | expression tag |
Gly 445 | - | expression tag |
Ala 545 | His 545 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Ala 1020 | Thr 20 | engineered mutation |
Asp 1024 | Asn 24 | engineered mutation |
Ala 1027 | Thr 27 | engineered mutation |
Thr 1028 | Ser 28 | engineered mutation |
Asp 1034 | Val 34 | engineered mutation |
Ile 1037 | Val 37 | engineered mutation |
Gly 1041 | Glu 41 | engineered mutation |
Glu 1057 | Lys 57 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 51471.5 | |
Non-Polymers* | Number of molecules | 2 |
Total formula weight | 130.8 | |
All* | Total formula weight | 51602.3 |