3G82

Complex of GS-alpha with the catalytic domains of mammalian adenylyl cyclase: complex with MANT-ITP and Mn

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Adenylate cyclase type 5	polymer	225	25526.5	1	UniProt (P30803) Pfam (PF00211) In PDB	Canis lupus familiaris (dogs)	Adenylate cyclase type V, ATP pyrophosphate-lyase 5, Adenylyl cyclase 5, Ca(2+)-inhibitable adenylyl cyclase
2	B	Adenylate cyclase type 2	polymer	212	23717.0	1	UniProt (P26769) Pfam (PF00211) In PDB	Rattus norvegicus (brown rat rat rats)	Adenylate cyclase type II, ATP pyrophosphate-lyase 2, Adenylyl cyclase 2
3	C	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	394	45769.5	1	UniProt (P04896) Pfam (PF00503) In PDB	Bos taurus (bovine cow domestic cattle domestic cow)	Adenylate cyclase-stimulating G alpha protein
4	A	MANGANESE (II) ION	non-polymer		54.9	2	Chemie (MN)
5	A	FORSKOLIN	non-polymer		410.5	1	Chemie (FOK)
6	A	3'-O-{[2-(methylamino)phenyl]carbonyl}inosine 5'-(tetrahydrogen triphosphate)	non-polymer		641.3	1	Chemie (MI3)
7	C	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
8	C	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
9	C	5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE	non-polymer		539.2	1	Chemie (GSP)
10		water	water		18.0	4	Chemie (HOH)

Sequence modifications

A: 363 - 580 (UniProt: P30803)

PDB	External Database	Details
Met 356	-	expression tag
His 357	-	expression tag
His 358	-	expression tag
His 359	-	expression tag
His 360	-	expression tag
His 361	-	expression tag
His 362	-	expression tag
Met 476	Val 557	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	95013.1
Non-Polymers*	Number of molecules	7
	Total formula weight	1760.7
All*	Total formula weight	96773.8

*Water molecules are not included.