3G4I

Crystal structure of human phosphodiesterase 4d with d155871

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	cAMP-specific 3',5'-cyclic phosphodiesterase 4D	polymer	381	43790.2	4	UniProt (Q08499) Pfam (PF00233) In PDB	Homo sapiens (human)	DPDE3, PDE43
2	A, B, C, D	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
3	A, B, C, D	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
4	A, B, C	SULFATE ION	non-polymer		96.1	3	Chemie (SO4)
5	A, B, C, D	1-(3-nitrophenyl)-3-(pyridin-4-ylmethyl)pyrido[2,3-d]pyrimidine-2,4(1H,3H)-dione	non-polymer		375.3	4	Chemie (D71)
6	D, A, B, C	ETHANOL	non-polymer		46.1	8	Chemie (EOH)
7		water	water		18.0	1203	Chemie (HOH)

Sequence modifications

A, B, C, D: 244 - 617 (UniProt: Q08499)

PDB	External Database	Details
Met 243	-	expression tag
Ala 579	Ser 715	engineered mutation
Ala 581	Ser 717	engineered mutation
His 618	-	expression tag
His 619	-	expression tag
His 620	-	expression tag
His 621	-	expression tag
His 622	-	expression tag
His 623	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	175160.7
Non-Polymers*	Number of molecules	23
	Total formula weight	2516.9
All*	Total formula weight	177677.6

*Water molecules are not included.