3G2D

Complex of Mth0212 and a 4 bp dsDNA with 3'-overhang

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Exodeoxyribonuclease	polymer	265	31429.7	2	UniProt (O26314) Pfam (PF03372)	Methanothermobacter thermautotrophicus	Mth0212
2	C, E (G, H)	5'-D(*CP*GP*CP*G*CP*AP*GP*GP*C)-3'	polymer	9	2741.8	2
3	D, F (K, I)	5'-D(*CP*CP*TP*GP*UP*GP*CP*GP*AP*T)-3'	polymer	10	3022.0	2
4	G, H, I (A)	PHOSPHATE ION	non-polymer		95.0	3	Chemie (PO4)
5	J, K, M, R (A, B)	TETRAETHYLENE GLYCOL	non-polymer		194.2	4	Chemie (PG4)
6	L, N, O, P, Q... (A, B, K)	GLYCEROL	non-polymer		92.1	7	Chemie (GOL)
7	U, V, W, X, Y... (A, B, G, K, H...)	water	water		18.0	313	Chemie (HOH)

Sequence modifications

A, B: 1 - 257 (UniProt: O26314)

PDB	External Database	Details
Ala 2	Thr 2	engineered mutation
Asn 151	Asp 151	engineered mutation
Leu 258	-	expression tag
Glu 259	-	expression tag
His 260	-	expression tag
His 261	-	expression tag
His 262	-	expression tag
His 263	-	expression tag
His 264	-	expression tag
His 265	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	74386.9
Non-Polymers*	Number of molecules	14
	Total formula weight	1706.5
All*	Total formula weight	76093.3

*Water molecules are not included.