3G00

Mth0212 in complex with a 9bp blunt end dsDNA at 1.7 Angstrom

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Exodeoxyribonuclease	polymer	265	31429.7	2	UniProt (O26314) Pfam (PF03372)	Methanothermobacter thermautotrophicus	Mth0212
2	C (H)	5'-D(*CP*GP*TP*AP*TP*TP*AP*CP*G)-3'	polymer	9	2730.8	1
3	D (I)	5'-D(*CP*GP*TP*AP*UP*TP*AP*CP*G)-3'	polymer	9	2716.8	1
4	E, F, J, M (A, B)	PHOSPHATE ION	non-polymer		95.0	4	Chemie (PO4)
5	G, H, K (A, B)	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
6	I, L (A, B)	(4S)-2-METHYL-2,4-PENTANEDIOL	non-polymer		118.2	2	Chemie (MPD)
7	N, O, P, Q (A, B, H, I)	water	water		18.0	659	Chemie (HOH)

Sequence modifications

A, B: 1 - 257 (UniProt: O26314)

PDB	External Database	Details
Ala 2	Thr 2	engineered mutation
Asn 151	Asp 151	engineered mutation
Leu 258	-	expression tag
Glu 259	-	expression tag
His 260	-	expression tag
His 261	-	expression tag
His 262	-	expression tag
His 263	-	expression tag
His 264	-	expression tag
His 265	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	68306.9
Non-Polymers*	Number of molecules	9
	Total formula weight	892.5
All*	Total formula weight	69199.4

*Water molecules are not included.