3FWH

Structure of haloalkane dehalogenase mutant Dha15 (I135F/C176Y) from Rhodococcus rhodochrous

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Haloalkane dehalogenase	polymer	299	34204.9	1	UniProt (P0A3G3) Pfam (PF00561) In PDB	Rhodococcus sp.
2	A	ACETATE ION	non-polymer		59.0	1	Chemie (ACT)
3	A	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
4	A	ISOPROPYL ALCOHOL	non-polymer		60.1	1	Chemie (IPA)
5		water	water		18.0	617	Chemie (HOH)

Sequence modifications

A: 1 - 293 (UniProt: P0A3G3)

PDB	External Database	Details
Phe 135	Ile 135	engineered mutation
Tyr 176	Cys 176	engineered mutation
His 294	-	expression tag
His 295	-	expression tag
His 296	-	expression tag
His 297	-	expression tag
His 298	-	expression tag
His 299	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	34204.9
Non-Polymers*	Number of molecules	4
	Total formula weight	190.0
All*	Total formula weight	34395.0

*Water molecules are not included.