3FTN

Q165E/S254K Double Mutant Chimera of alcohol dehydrogenase by exchange of the cofactor binding domain res 153-295 of T. brockii ADH by C. beijerinckii ADH

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	NADP-dependent alcohol dehydrogenase	polymer	352	37854.2	4	UniProt (P14941) UniProt (P25984) Pfam (PF08240) Pfam (PF00107) In PDB	Thermoanaerobacter brockii
2	D, A, B, C	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
3	A, B, C	ACETATE ION	non-polymer		59.0	4	Chemie (ACT)
4	C, A, B	1,2-ETHANEDIOL	non-polymer		62.1	11	Chemie (EDO)
5	D, A, B, C	CHLORIDE ION	non-polymer		35.5	6	Chemie (CL)
6		water	water		18.0	905	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 152 (UniProt: P14941)
A, B, C, D: 153 - 295 (UniProt: P25984)

PDB	External Database	Details
Glu 165	Gln 165	engineered mutation
Lys 254	Ser 254	engineered mutation

A, B, C, D: 296 - 352 (UniProt: P14941)

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	151416.8
Non-Polymers*	Number of molecules	25
	Total formula weight	1393.3
All*	Total formula weight	152810.1

*Water molecules are not included.