3FSU

Crystal Structure of Escherichia coli Methylenetetrahydrofolate Reductase Double Mutant Phe223LeuGlu28Gln complexed with methyltetrahydrofolate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, C, E)	5,10-methylenetetrahydrofolate reductase	polymer	304	34180.9	3	UniProt (P0AEZ1) Pfam (PF02219)	Escherichia coli K-12
2	D, F, H (A, C, E)	FLAVIN-ADENINE DINUCLEOTIDE	non-polymer		785.5	3	Chemie (FAD)
3	E, I (A, E)	5-METHYL-5,6,7,8-TETRAHYDROFOLIC ACID	non-polymer		459.5	2	Chemie (C2F)
4	G (C)	MESO-ERYTHRITOL	non-polymer		122.1	1	Chemie (MRY)
5	J (E)	SULFATE ION	non-polymer		96.1	1	Chemie (SO4)
6	K, L, M (A, C, E)	water	water		18.0	400	Chemie (HOH)

Sequence modifications

A, C, E: 1 - 296 (UniProt: P0AEZ1)

PDB	External Database	Details
Gln 28	Glu 28	engineered mutation
Leu 223	Phe 223	engineered mutation
Leu 297	-	expression tag
Glu 298	-	expression tag
His 299	-	expression tag
His 300	-	expression tag
His 301	-	expression tag
His 302	-	expression tag
His 303	-	expression tag
His 304	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	102542.6
Non-Polymers*	Number of molecules	7
	Total formula weight	3493.7
All*	Total formula weight	106036.3

*Water molecules are not included.