3FST

Crystal Structure of Escherichia coli Methylenetetrahydrofolate Reductase Mutant Phe223Leu at pH 7.4

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, C, E)	5,10-methylenetetrahydrofolate reductase	polymer	304	34181.8	3	UniProt (P0AEZ1) Pfam (PF02219)	Escherichia coli K-12
2	D, E, G (A, C, E)	FLAVIN-ADENINE DINUCLEOTIDE	non-polymer		785.5	3	Chemie (FAD)
3	F (C)	MESO-ERYTHRITOL	non-polymer		122.1	1	Chemie (MRY)
4	H, I, J (E)	SULFATE ION	non-polymer		96.1	3	Chemie (SO4)
5	K, L, M (A, C, E)	water	water		18.0	456	Chemie (HOH)

Sequence modifications

A, C, E: 1 - 296 (UniProt: P0AEZ1)

PDB	External Database	Details
Leu 223	Phe 223	engineered mutation
Leu 297	-	expression tag
Glu 298	-	expression tag
His 299	-	expression tag
His 300	-	expression tag
His 301	-	expression tag
His 302	-	expression tag
His 303	-	expression tag
His 304	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	102545.5
Non-Polymers*	Number of molecules	7
	Total formula weight	2767.0
All*	Total formula weight	105312.5

*Water molecules are not included.