3FST
Crystal Structure of Escherichia coli Methylenetetrahydrofolate Reductase Mutant Phe223Leu at pH 7.4
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, C, E | 5,10-methylenetetrahydrofolate reductase | polymer | 304 | 34181.8 | 3 | UniProt (P0AEZ1) Pfam (PF02219) In PDB | Escherichia coli K-12 | |
2 | A, C, E | FLAVIN-ADENINE DINUCLEOTIDE | non-polymer | 785.5 | 3 | Chemie (FAD) | |||
3 | C | MESO-ERYTHRITOL | non-polymer | 122.1 | 1 | Chemie (MRY) | |||
4 | E | SULFATE ION | non-polymer | 96.1 | 3 | Chemie (SO4) | |||
5 | water | water | 18.0 | 456 | Chemie (HOH) |
Sequence modifications
A, C, E: 1 - 296 (UniProt: P0AEZ1)
PDB | External Database | Details |
---|---|---|
Leu 223 | Phe 223 | engineered mutation |
Leu 297 | - | expression tag |
Glu 298 | - | expression tag |
His 299 | - | expression tag |
His 300 | - | expression tag |
His 301 | - | expression tag |
His 302 | - | expression tag |
His 303 | - | expression tag |
His 304 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 3 |
Total formula weight | 102545.5 | |
Non-Polymers* | Number of molecules | 7 |
Total formula weight | 2767.0 | |
All* | Total formula weight | 105312.5 |