3FLC

Crystal structure of the His-tagged H232R mutant of glycerol kinase from Enterococcus casseliflavus with glycerol

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (O, X)	Glycerol kinase	polymer	518	57237.1	2	UniProt (O34153) Pfam (PF00370) Pfam (PF02782)	Enterococcus casseliflavus	ATP:glycerol 3-phosphotransferase, Glycerokinase, GK
2	C, E (O, X)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
3	D, F (O, X)	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
4	G, H (O, X)	water	water		18.0	708	Chemie (HOH)

Sequence modifications

O, X: 1 - 506 (UniProt: O34153)

PDB	External Database	Details
Met -11	-	expression tag
Arg -10	-	expression tag
Gly -9	-	expression tag
Ser -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Arg 232	His 232	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	114474.1
Non-Polymers*	Number of molecules	4
	Total formula weight	376.3
All*	Total formula weight	114850.5

*Water molecules are not included.