3FLC

Crystal structure of the His-tagged H232R mutant of glycerol kinase from Enterococcus casseliflavus with glycerol

Functional Information from GO Data

Chain	GOid	namespace	contents
O	0000166	molecular_function	nucleotide binding
O	0004370	molecular_function	glycerol kinase activity
O	0005524	molecular_function	ATP binding
O	0005829	cellular_component	cytosol
O	0005975	biological_process	carbohydrate metabolic process
O	0006071	biological_process	glycerol metabolic process
O	0006072	biological_process	glycerol-3-phosphate metabolic process
O	0016301	molecular_function	kinase activity
O	0016740	molecular_function	transferase activity
O	0016773	molecular_function	phosphotransferase activity, alcohol group as acceptor
O	0019563	biological_process	glycerol catabolic process
X	0000166	molecular_function	nucleotide binding
X	0004370	molecular_function	glycerol kinase activity
X	0005524	molecular_function	ATP binding
X	0005829	cellular_component	cytosol
X	0005975	biological_process	carbohydrate metabolic process
X	0006071	biological_process	glycerol metabolic process
X	0006072	biological_process	glycerol-3-phosphate metabolic process
X	0016301	molecular_function	kinase activity
X	0016740	molecular_function	transferase activity
X	0016773	molecular_function	phosphotransferase activity, alcohol group as acceptor
X	0019563	biological_process	glycerol catabolic process

Functional Information from PDB Data

site_id	AC1
Number of Residues	9
Details	BINDING SITE FOR RESIDUE GOL O 507

Chain	Residue
O	ARG84
O	GLU85
O	TRP104
O	TYR136
O	ASP246
O	GLN247
O	PHE271
O	SO4508
O	HOH622

---

site_id	AC2
Number of Residues	9
Details	BINDING SITE FOR RESIDUE SO4 O 508

Chain	Residue
O	GLY13
O	THR14
O	THR15
O	GLY267
O	THR268
O	GOL507
O	HOH558
O	HOH622
O	HOH771

---

site_id	AC3
Number of Residues	9
Details	BINDING SITE FOR RESIDUE GOL X 507

Chain	Residue
X	ARG84
X	GLU85
X	TRP104
X	TYR136
X	ASP246
X	GLN247
X	PHE271
X	SO4508
X	HOH688

---

site_id	AC4
Number of Residues	8
Details	BINDING SITE FOR RESIDUE SO4 X 508

Chain	Residue
X	THR14
X	GLY267
X	THR268
X	GOL507
X	HOH562
X	HOH688
X	HOH816
X	HOH845

---

Functional Information from PROSITE/UniProt

site_id	PS00445
Number of Residues	21
Details	FGGY_KINASES_2 FGGY family of carbohydrate kinases signature 2. GaVFGLtrgttke.DFVRATLQ

Chain	Residue	Details
O	GLY363-GLN383

---

site_id	PS00933
Number of Residues	13
Details	FGGY_KINASES_1 FGGY family of carbohydrate kinases signature 1. YfSatKVRWLLDN

Chain	Residue	Details
O	TYR136-ASN148

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	26
Details	Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_00186","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_00186","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"19102629","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"3D7E","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	Modified residue: {"description":"Phosphohistidine; by HPr","evidences":[{"source":"HAMAP-Rule","id":"MF_00186","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"9162046","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

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