3FJ4

Crystal structure of muconate lactonizing enzyme from Pseudomonas Fluorescens complexed with muconolactone

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Muconate cycloisomerase	polymer	382	41051.9	2	UniProt (Q4K9X1) Pfam (PF02746) Pfam (PF13378)	Pseudomonas fluorescens
2	C, E (A, B)	[(2S)-5-oxo-2,5-dihydrofuran-2-yl]acetic acid	non-polymer		142.1	2	Chemie (MUC)
3	D, F (A, B)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
4	G, H (A, B)	water	water		18.0	454	Chemie (HOH)

Sequence modifications

A, B: 4 - 374 (UniProt: Q4K9X1)

PDB	External Database	Details
Met 1	-	expression tag
Ser 2	-	expression tag
Leu 3	-	expression tag
Glu 375	-	expression tag
Gly 376	-	expression tag
His 377	-	expression tag
His 378	-	expression tag
His 379	-	expression tag
His 380	-	expression tag
His 381	-	expression tag
His 382	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	82103.9
Non-Polymers*	Number of molecules	4
	Total formula weight	332.8
All*	Total formula weight	82436.7

*Water molecules are not included.