3FJ4
Crystal structure of muconate lactonizing enzyme from Pseudomonas Fluorescens complexed with muconolactone
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0006518 | biological_process | peptide metabolic process |
| A | 0009063 | biological_process | amino acid catabolic process |
| A | 0016853 | molecular_function | isomerase activity |
| A | 0016854 | molecular_function | racemase and epimerase activity |
| A | 0018849 | molecular_function | muconate cycloisomerase activity |
| A | 0018850 | molecular_function | chloromuconate cycloisomerase activity |
| A | 0030145 | molecular_function | manganese ion binding |
| A | 0046872 | molecular_function | metal ion binding |
| B | 0006518 | biological_process | peptide metabolic process |
| B | 0009063 | biological_process | amino acid catabolic process |
| B | 0016853 | molecular_function | isomerase activity |
| B | 0016854 | molecular_function | racemase and epimerase activity |
| B | 0018849 | molecular_function | muconate cycloisomerase activity |
| B | 0018850 | molecular_function | chloromuconate cycloisomerase activity |
| B | 0030145 | molecular_function | manganese ion binding |
| B | 0046872 | molecular_function | metal ion binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 13 |
| Details | BINDING SITE FOR RESIDUE MUC A 383 |
| Chain | Residue |
| A | HIS24 |
| A | LYS275 |
| A | THR303 |
| A | GLU329 |
| A | MG1001 |
| A | ILE56 |
| A | TYR61 |
| A | THR143 |
| A | LYS169 |
| A | LYS171 |
| A | ASP200 |
| A | ASN202 |
| A | ASP251 |
| site_id | AC2 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE MG A1001 |
| Chain | Residue |
| A | ASP200 |
| A | ASN202 |
| A | GLU226 |
| A | ASP251 |
| A | MUC383 |
| A | HOH503 |
| site_id | AC3 |
| Number of Residues | 15 |
| Details | BINDING SITE FOR RESIDUE MUC B 383 |
| Chain | Residue |
| B | HIS24 |
| B | ILE56 |
| B | TYR61 |
| B | THR143 |
| B | LYS169 |
| B | LYS171 |
| B | ASP200 |
| B | ASN202 |
| B | ASP251 |
| B | LYS275 |
| B | THR303 |
| B | GLU329 |
| B | PHE331 |
| B | HOH502 |
| B | MG1002 |
| site_id | AC4 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE MG B1002 |
| Chain | Residue |
| B | ASP200 |
| B | ASN202 |
| B | GLU226 |
| B | ASP251 |
| B | MUC383 |
| B | HOH501 |
| B | HOH502 |
Functional Information from PROSITE/UniProt
| site_id | PS00908 |
| Number of Residues | 26 |
| Details | MR_MLE_1 Mandelate racemase / muconate lactonizing enzyme family signature 1. AkSGIEsALlDAqGKrlglPVseLLG |
| Chain | Residue | Details |
| A | ALA106-GLY131 |
| site_id | PS00909 |
| Number of Residues | 32 |
| Details | MR_MLE_2 Mandelate racemase / muconate lactonizing enzyme family signature 2. VrvDvNqawdeavAlracriLggngidlIEQP |
| Chain | Residue | Details |
| A | VAL197-PRO228 |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1muc |
| Chain | Residue | Details |
| A | GLU329 | |
| A | LYS169 | |
| A | LYS171 |
| site_id | CSA2 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1muc |
| Chain | Residue | Details |
| B | GLU329 | |
| B | LYS169 | |
| B | LYS171 |
| site_id | CSA3 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1muc |
| Chain | Residue | Details |
| A | LYS275 | |
| A | LYS171 |
| site_id | CSA4 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1muc |
| Chain | Residue | Details |
| B | LYS275 | |
| B | LYS171 |
| site_id | MCSA1 |
| Number of Residues | 8 |
| Details | M-CSA 269 |
| Chain | Residue | Details |
| A | HIS24 | steric role |
| A | LYS169 | electrostatic stabiliser |
| A | LYS171 | electrostatic stabiliser, proton donor |
| A | ASP200 | metal ligand |
| A | GLU226 | metal ligand |
| A | ASP251 | metal ligand |
| A | LYS275 | electrostatic stabiliser |
| A | GLU329 | proton acceptor, proton donor |
| site_id | MCSA2 |
| Number of Residues | 8 |
| Details | M-CSA 269 |
| Chain | Residue | Details |
| B | HIS24 | steric role |
| B | LYS169 | electrostatic stabiliser |
| B | LYS171 | electrostatic stabiliser, proton donor |
| B | ASP200 | metal ligand |
| B | GLU226 | metal ligand |
| B | ASP251 | metal ligand |
| B | LYS275 | electrostatic stabiliser |
| B | GLU329 | proton acceptor, proton donor |
