3FCZ

Adaptive protein evolution grants organismal fitness by improving catalysis and flexibility

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Beta-lactamase 2	polymer	222	24371.8	2	UniProt (P04190) Pfam (PF00753) In PDB	Bacillus cereus	Beta-lactamase II, Penicillinase, Cephalosporinase
2	A, B	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
3		water	water		18.0	42	Chemie (HOH)

Sequence modifications

A, B: 32 - 281 (UniProt: P04190)

PDB	External Database	Details
Ser 70	Asn 72	engineered mutation
Ala 112	Val 112	engineered mutation
Ser 250	Leu 227	engineered mutation
Ser 262	Gly 239	engineered mutation
Leu 282	-	expression tag
Leu 283	-	expression tag
Leu 284	-	expression tag
His 285	-	expression tag
Thr 286	-	expression tag
Leu 287	-	expression tag
Asp 288	-	expression tag
Leu 289	-	expression tag
Leu 290	-	expression tag
Lys 291	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	48743.5
Non-Polymers*	Number of molecules	4
	Total formula weight	261.6
All*	Total formula weight	49005.2

*Water molecules are not included.