3FCZ
Adaptive protein evolution grants organismal fitness by improving catalysis and flexibility
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.7 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.670, 48.810, 77.190 |
| Unit cell angles | 90.00, 109.86, 90.00 |
Refinement procedure
| Resolution | 72.599 - 2.804 |
| R-factor | 0.197 |
| Rwork | 0.194 |
| R-free | 0.25810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bc2 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.777 |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 100.000 |
| High resolution limit [Å] | 2.800 |
| Rmerge | 0.103 |
| Number of reflections | 8961 |
| <I/σ(I)> | 11.2 |
| Completeness [%] | 73.6 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 24% PEG 3350, 0.1M sodium tartrate, 1mM DTT, 1mM zinc acetate, 0.1M sodium cacodylate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
