3F5J
Semi-active E176Q mutant of rice BGlu1, a plant exoglucanase/beta-glucosidase
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Beta-glucosidase | polymer | 481 | 54727.5 | 2 | UniProt (Q42975) Pfam (PF00232) UniProt (by SIFTS) (Q75I93) | Oryza sativa Japonica Group (Japanese rice) | |
| 2 | C, D (C, D) | beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-beta-D-glucopyranose | branched | 666.6 | 2 | In PDB BIRD (PRD_900011) GlyTouCan (G00025MO) | beta-cellotetraose | ||
| 3 | E (A) | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) | |||
| 4 | F, H (A, B) | SULFATE ION | non-polymer | 96.1 | 2 | Chemie (SO4) | |||
| 5 | G, I (A, B) | 2-(N-MORPHOLINO)-ETHANESULFONIC ACID | non-polymer | 195.2 | 2 | Chemie (MES) | |||
| 6 | J, K (A, B) | water | water | 18.0 | 727 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 476 (UniProt: Q42975)
| PDB | External Database | Details |
|---|---|---|
| Ala -4 | - | expression tag |
| Met -3 | - | expression tag |
| Ala -2 | - | expression tag |
| Asp -1 | - | expression tag |
| Val 0 | - | expression tag |
| Gln 176 | Glu 204 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 109455.0 | |
| Branched | Number of molecules | 2 |
| Total formula weight | 1333.2 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 648.0 | |
| All* | Total formula weight | 111436.1 |
