3F5J
Semi-active E176Q mutant of rice BGlu1, a plant exoglucanase/beta-glucosidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13C1 |
| Synchrotron site | NSRRC |
| Beamline | BL13C1 |
| Temperature [K] | 105 |
| Detector technology | CCD |
| Collection date | 2007-03-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 79.731, 100.843, 127.472 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.840 - 1.950 |
| R-factor | 0.17993 |
| Rwork | 0.178 |
| R-free | 0.21365 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cbg |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.585 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.083 | 0.292 |
| Number of reflections | 73603 | |
| <I/σ(I)> | 19.18 | 6.91 |
| Completeness [%] | 97.4 | 93.7 |
| Redundancy | 6.3 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 288 | 22% PEG MME 5000, 0.18M ammonium sulfate, 0.1M MES, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
