3EZJ
Crystal structure of the N-terminal domain of the secretin GspD from ETEC determined with the assistance of a nanobody
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, C, E, G) | General secretion pathway protein GspD | polymer | 241 | 26227.7 | 4 | UniProt (B3IFK0) Pfam (PF21305) Pfam (PF03958) UniProt (by SIFTS) (Q707C1) | Escherichia coli | |
| 2 | E, F, G, H (B, D, F, H) | NANOBODY NBGSPD_7 | polymer | 126 | 13748.1 | 4 | LAMA GLAMA (llama) | ||
| 3 | I, J, K, L, M... (A, C, E, G, B...) | PHOSPHATE ION | non-polymer | 95.0 | 8 | Chemie (PO4) | |||
| 4 | N, P, R, T (B, D, F, H) | CHLORIDE ION | non-polymer | 35.5 | 4 | Chemie (CL) | |||
| 5 | AA, BA, U, V, W... (F, H, A, C, E...) | water | water | 18.0 | 264 | Chemie (HOH) |
Sequence modifications
A, C, E, G: 0 - 237 (UniProt: B3IFK0)
| PDB | External Database | Details |
|---|---|---|
| Gly -3 | - | expression tag |
| Ala -2 | - | expression tag |
| Met -1 | - | expression tag |
| Ala 38 | Lys 78 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 159903.4 | |
| Non-Polymers* | Number of molecules | 12 |
| Total formula weight | 901.6 | |
| All* | Total formula weight | 160805.0 |
