3EZJ
Crystal structure of the N-terminal domain of the secretin GspD from ETEC determined with the assistance of a nanobody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-05-08 |
| Detector | MARMOSAIC 325 mm CCD |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 98.520, 98.520, 402.100 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.670 - 2.800 |
| R-factor | 0.194 |
| Rwork | 0.192 |
| R-free | 0.23900 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.063 |
| Data scaling software | XSCALE |
| Phasing software | SHELX |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.400 | 2.800 |
| Rmerge | 0.086 | 0.767 |
| Number of reflections | 86152 | |
| <I/σ(I)> | 2.2 | |
| Completeness [%] | 91.7 | 91.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 298 | 1.0M NA/K PHOSPHATE, pH 5.0, vapor diffusion, sitting drop, temperature 298K |
