3EZ5
Cocrystal structure of Bacillus fragment DNA polymerase I with duplex DNA , dCTP, and zinc (closed form).
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, D | DNA polymerase I | polymer | 580 | 66202.9 | 2 | Pfam (PF22619) Pfam (PF00476) UniProt (by SIFTS) (P52026) In PDB | Bacillus stearothermophilus | |
2 | B, E | 5'-D(*DCP*DCP*DTP*DGP*DAP*DCP*DTP*DCP*DG)-3' | polymer | 9 | 2691.8 | 2 | |||
3 | C, F | 5'-D(*DAP*DTP*DTP*DCP*DGP*DAP*DGP*DTP*DCP*DAP*DGP*DG)-3' | polymer | 12 | 3702.4 | 2 | |||
4 | G, H | beta-D-fructofuranose-(2-1)-alpha-D-glucopyranose | branched | 342.3 | 2 | In PDB BIRD (PRD_900003) | sucrose | ||
5 | A, D | SULFATE ION | non-polymer | 96.1 | 3 | Chemie (SO4) | |||
6 | A, D | ZINC ION | non-polymer | 65.4 | 5 | Chemie (ZN) | |||
7 | A, D | 2',3'-DIDEOXYADENOSINE-5'-TRIPHOSPHATE | non-polymer | 475.2 | 2 | Chemie (DAD) | |||
8 | water | water | 18.0 | 540 | Chemie (HOH) |
Sequence modifications
A, D: 297 - 876 (PDB: 3EZ5)
PDB | External Database | Details |
---|---|---|
Tyr 710 | Phe 414 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 6 |
Total formula weight | 145194.3 | |
Branched | Number of molecules | 2 |
Total formula weight | 684.6 | |
Non-Polymers* | Number of molecules | 10 |
Total formula weight | 1565.6 | |
All* | Total formula weight | 147444.5 |