3EZ5
Cocrystal structure of Bacillus fragment DNA polymerase I with duplex DNA , dCTP, and zinc (closed form).
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-10-21 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.282420 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 92.551, 108.572, 149.756 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.830 - 1.900 |
R-factor | 0.212 |
Rwork | 0.211 |
R-free | 0.24400 |
Starting model (for MR) | 2hvi |
RMSD bond length | 0.010 |
RMSD bond angle | 1.382 |
Data scaling software | XSCALE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.058 | 0.398 |
Number of reflections | 119138 | 32718 |
<I/σ(I)> | 12.28 | 3.4 |
Completeness [%] | 99.9 | 100 |
Redundancy | 3.78 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 290 | Ammonium Sulfate, MES, MPD, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | Ammonium Sulfate | ||
2 | 1 | 1 | MES | ||
3 | 1 | 1 | MPD | ||
4 | 1 | 2 | Ammonium Sulfate | ||
5 | 1 | 2 | MSE | ||
6 | 1 | 2 | MPD |