3EX7

The crystal structure of EJC in its transition state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E (A, E)	Protein mago nashi homolog	polymer	146	17189.6	2	UniProt (P61326) Pfam (PF02792)	Homo sapiens (human)
2	B, F (B, G)	RNA-binding protein 8A	polymer	126	14637.2	2	UniProt (Q9Y5S9) Pfam (PF00076)	Homo sapiens (human)	RNA-binding motif protein 8A, Ribonucleoprotein RBM8A, RNA-binding protein Y14, Binder of OVCA1-1, BOV-1
3	C, G (C, H)	Eukaryotic initiation factor 4A-III	polymer	413	47173.2	2	UniProt (P38919) Pfam (PF00270) Pfam (PF00271)	Homo sapiens (human)	Eukaryotic translation initiation factor 4A isoform 3, ATP-dependent RNA helicase eIF4A-3, ATP-dependent RNA helicase DDX48, DEAD box protein 48, Eukaryotic initiation factor 4A-like NUK-34, Nuclear matrix protein 265, hNMP 265
4	D, H (D, I)	Protein CASC3	polymer	146	17275.1	2	UniProt (O15234) Pfam (PF09405)	Homo sapiens (human)	Cancer susceptibility candidate gene 3 protein, Metastatic lymph node protein 51, Protein MLN 51, Protein barentsz, Btz
5	I, J (F, J)	RNA (5'-R(*UP*UP*UP*UP*UP*U)-3')	polymer	6	1792.0	2
6	K, N (C, H)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	2	Chemie (ADP)
7	L, O (C, H)	ALUMINUM FLUORIDE	non-polymer		84.0	2	Chemie (AF3)
8	M, P (C, H)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
9	Q, R, S, T, U... (A, B, C, D, E...)	water	water		18.0	380	Chemie (HOH)

Sequence modifications

B, G: 51 - 174 (UniProt: Q9Y5S9)

PDB	External Database	Details
Leu 175	-	expression tag
Glu 176	-	expression tag

C, H: 1 - 411 (UniProt: P38919)

PDB	External Database	Details
Leu 412	-	expression tag
Glu 413	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	196134.3
Non-Polymers*	Number of molecules	6
	Total formula weight	1071.0
All*	Total formula weight	197205.2

*Water molecules are not included.